Multiple resistance factors collectively promote inoculum-dependent dynamic survival during antimicrobial peptide exposure in Enterobacter cloacae
Fig 2
E. cloacae exhibits population heterogeneity in its response to cecropin B.
(A) Cells were grown to mid-exponential phase in liquid medium and then placed on 0.8% agarose pads containing the indicated amounts of cecropin and incubated at 37°C. Representative, cropped frames from a time series are shown. In the 20 μg/mL condition, red arrows indicate examples of lysing cells. (B) Percentage of cells that divided during the time lapse, grouped by drug concentration (0, 10, 20, 200 μg/mL). Cells are binned by birth frame, i.e., the frame of the time lapse during which the cell was first identified by SuperSegger [85]. Blue represents cells that underwent a successful division event throughout the course of the timelapse, red indicates cells which did not divide. Missing bars indicate that the initial population of cells was unable to divide.