Multiple resistance factors collectively promote inoculum-dependent dynamic survival during antimicrobial peptide exposure in Enterobacter cloacae
Fig 1
E. cloacae displays inoculum-dependent dynamic survival in the presence of cecB.
Overnight cultures were diluted (A) 10-fold or (B) 100-fold into fresh LB containing 20 μg/mL cecB, and cells were grown at 37°C for 24 hours. OD600 measurements were taken every 5 minutes. Error bars represent standard deviation (n = 3). (C) Cultures were prepared as in (B), but samples were taken at indicated timepoints to quantify colony forming units (CFU) per mL. Error bars represent standard deviation (n = 3). (D) Inoculum effect in different growth media. MIC assays were conducted at the indicated dilution of an overnight culture of E. cloacae.