A targeted CRISPR screen identifies ETS1 as a regulator of HIV-1 latency
Fig 7
Analysis of differential gene expression in CD4 T cells from PWH on ART after depletion of ETS1.
CD4 T cells from three PWH on ART were nucleofected with Cas9/gRNA complexes targeting ETS1 or non-targeting control (NT) as described in Fig 6, followed by bulk RNAseq. (A) Principal component analysis (PCA) plot of samples based on gene expression data for each sample. ETS1-targeted samples shown by blue dots, non-targeting gRNA samples by red dots. Each data point represents an individual sample. The y-axis and x-axis represent the first and second principal components, respectively. (B) Volcano plot of overall differentially expressed genes (DEGs) between HIV-1 infected CD4 T cells nucleofected with ETS1 targeting sgRNA/Ca9 complexes vs non-targeting. (C) Database for Annotation, Visualization and Integrated Discovery (DAVID) analysis of upregulated and downregulated genes of the RNA-sequencing analysis of ETS1 vs control cells. The 10 most significant KEGG pathways are shown.