A targeted CRISPR screen identifies ETS1 as a regulator of HIV-1 latency
Fig 2
CRISPR Screen of TxLatent library.
Latency HIV-CRISPR screen data is displayed for J-Lat 10.6 cells transduced with TxLatent library and subsequently treated with DMSO (A), AZD5582 (10nM) (B), prostratin (75nM) (C), iBET151 (75nM) (D), or vorinostat (500nM) (E). Genes in the TxLatent library are randomly displayed on the x-axis, in the same order across each treatment. The -Log(MAGeCK Score), a statistical pipeline that takes into account the enrichment/depletion of all guides for a given gene as well as the variation between replicates [16] is plotted for the enriched genes and the Log(MAGeCK score) is plotted for depleted genes, and combined together to display on a single graph for each treatment. A list of “synthetic” NTCs (synNTCs) was created by randomly grouping sets of 8 NTCs and used to generate a cutoff for gene hits that were enriched or depleted. SynNTCs are shown with white circles and genes targeted by gRNAs in the TxLatent library are shown with gray circles. The red lines on each graph represent the two standard deviations of mean of the synNTCs: DMSO, 1.52 and -1.91; AZD5582, 1.53 and -2.10; iBET151, 1.75 and -1.87; prostratin, 1.58 and -2.19; and vorinostat, 1.61 and -1.9.