Winners vs. losers: Schistosoma mansoni intestinal and liver eggs exhibit striking differences in gene expression and immunogenicity
Fig 5
Immunolocalization of IPSE/alpha-1 in mouse liver (A, C) and intestine sections (B, D) infected with S. mansoni.
(A, B) Overview axioscan images display entire sections of infected tissue, with three highlighted zoomed areas. (C, D) Confocal close-ups illustrate mature eggs with developed miracidia. Labels are represented by red for anti-IPSE/alpha-1 antibody-specific sites, green for eggshell autofluorescence, and blue for DAPI-labeled cell nuclei. The red anti-IPSE/alpha-1 signal is particularly intense in the subshell area and around liver eggs, while it is weaker or absent in the intestinal eggs. Scale bars: whole-organ section = 1000 μm, zoomed square areas = 50 μm, and confocal close-ups = 20 μm. For negative control images see S2 Fig.