A genome-wide CRISPR/Cas9 knockout screen identifies TMEM239 as an important host factor in facilitating African swine fever virus entry into early endosomes
Fig 5
Proteomic analysis of host proteins interacting with TMEM239.
(A) Detection of exogenous TMEM239 expression in WSL cells and visualization of proteins that interact with TMEM239 by using silver staining. pTMEM239-Flag (6μg) and empty pCAGGS-Flag (6μg) were transfected into WSL cells, respectively. At 24 hpt, ad-HRB1 was inoculated at MOI = 5 into one of the groups of cells transfected with TMEM239 plasmid. Anti-Flag (M2) agarose beads were used to pull down TMEM239 and its binding proteins 24 h after transfection. (B) Gene ontology (GO) analysis based on the interaction between TMEM239 and its binding proteins in the uninfected virus group. The GO distributions of all proteins were divided into three types (biological processes, composition category, and function category). (C) KEGG pathway enrichment analysis based on the interaction between TMEM239 and its binding proteins in the uninfected virus group. Cellular processes, Environmental information processing, and organismal systems are listed.