Sensitive detection of pathological seeds of α-synuclein, tau and prion protein on solid surfaces
Fig 6
sfRT-QuIC of stainless-steel bound brain homogenate derived human, cervid, ovine, and bovine prions.
Panel A. sfRT-QuIC of diluted SM collections from sCJD (MM1 type) or Alzheimer’s disease (as a prion-negative control) spots on a stainless-steel plate: average ThT fluorescence of 4 replicate wells versus reaction time. Panel B. sfRT-QuIC results from panel A represented as 1/time to threshold for each reaction seeded with the designated dilutions (log10 tissue equivalents; x-axis) as described initially in legend of Fig 4B and 4D. Panel C. Analogous data from plates contaminated with 10% BH from humans with variant Creutzfeldt-Jakob disease (vCJD), fatal familial insomnia (FFI), genetic Creutzfeldt-Jakob disease (gCJD; E200K), and Gerstmann-Sträussler-Scheinker disease (GSS; P102L); cervids with CWD; sheep with scrapie; cows with classical (C-), high- (H-) and low- (L-) BSE; and species-matched uninfected controls.