Sensitive detection of pathological seeds of α-synuclein, tau and prion protein on solid surfaces
Fig 5
sfRT-QuIC detection of seeding activity from different strains of prion-infected brain homogenates.
Panel A. Designated dilutions of sCJD (MM1 type) and sAD (prion negative control) BHs tested by solution (left) or sfRT-QuIC (right). Curves are show mean ThT fluorescence from quadruplicate wells versus assay duration. Panel B. 1/time to threshold comparisons of solution and sfRT-QuIC data from dilutions of sCJD (MM1 subtype; extracted from data shown in A), genetic Creutzfeldt-Jakob disease (gCJD; E200K), Gerstmann-Sträussler-Scheinker disease (GSS; P102L), ovine classical scrapie and CWD and uninfected controls (Ctrl) BHs. X-axis indicates the dilution (log10 tissue equivalents). Panel C. Table of Log SD50/mg original tissue values estimated using Spearman-Karber analysis of the data shown in Panel B as described in Methods. Comparable results were seen in analogous experiments performed with sCJD, ovine classical scrapie and CWD, except for the inclusion of 0.1% SDS in the brain homogenate diluent (data available on request).