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Sensitive detection of pathological seeds of α-synuclein, tau and prion protein on solid surfaces

Fig 2

sfRT-QuIC experimental design schematic.

Panel A. Spotting of surfaces with serially diluted brain homogenate (BH, yellow liquid; BE: brain equivalents) followed by sfRT-QuIC testing of sampling medium (SM; blue liquid). Panel B. Spotting of surfaces with 10% BH followed by serial dilutions of the SM for sfRT-QuIC. Panel C. sfRT-QuIC of surgical instruments contaminated with CJD-infected humanized mouse brain tissue: sampling procedure and serial dilutions of the SM for sfRT-QuIC. Created with BioRender.com.

Fig 2

doi: https://doi.org/10.1371/journal.ppat.1012175.g002