Merkel cell polyomavirus protein ALTO modulates TBK1 activity to support persistent infection
Fig 5
MCPyV rescues STING levels to stimulate IFN signaling.
A. Normal HDFs mock-infected or infected with MCPyV were stimulated with diABZI (or DMSO control) at the time FBS was added (2 days post-infection). At 5 days post-infection, cells were harvested, and mRNA levels were quantified by RT-qPCR. The value for mock-infected and DMSO-treated HDFs was set as 1. Error bars indicate standard deviation from 3 replicates. B. Normal HDFs were mock-infected or infected with MCPyV, then stimulated with diABZI (or DMSO control) at the time FBS was added (2 days post-infection). Whole cell lysates were collected at 5 days post-infection, resolved by SDS/PAGE, and immunoblotted with the indicated antibodies. C. Normal HDFs were mock-infected or infected with MCPyV, then stimulated with diABZI (or DMSO control) at the time FBS was added (2 days post-infection). Cells were fixed at 5 days post-infection and immunostained for LT and STING, and counterstained with DAPI. Scale bar, 20 μm.