Merkel cell polyomavirus protein ALTO modulates TBK1 activity to support persistent infection
Fig 2
ALTO stimulates TBK1S172 autophosphorylation and STING stabilization.
A. HDF-inALTO or -inRFP cells were mock-treated or induced with Dox for 24 hours, then stimulated with diABZI (or DMSO control) for 16 hours. Whole cell lysates were resolved by SDS/PAGE and immunoblotted with the indicated antibodies. B. HDF-inALTO or -inRFP cells were mock-treated or induced with Dox for 7 hours, then stimulated with diABZI (or DMSO control) for an additional 19 hours. Cells were fixed and immune-stained for STING, and counterstained with DAPI. Scale bar, 20 μm. C. HDF-inALTO or -inRFP were mock-treated or induced with Dox for 40 hours, then stimulated with diABZI (or DMSO control) for the indicated times. Whole cell lysates were resolved by SDS/PAGE and immunoblotted with indicated antibodies. Red asterisks indicate phosphorylated STING.