Sja-let-7 suppresses the development of liver fibrosis via Schistosoma japonicum extracellular vesicles
Fig 6
Sja-let-7 reduced the expression of fibrotic markers after treated with sja-let-7 agomir.
(A-C) Detection of α-SMA, Col1α1 and Col1α3 mRNA expression in the liver (n = 6). (D) Liver IHC analysis of α-SMA, Col1α1 and Col1α3. Scale bar, 200 μm. Insets show a higher magnification of the outlined area. Scale bar, 50 μm. (E) Immunofluorescence analysis of α-SMA, Col1α1 and Col1α3 after treated with sja-let-7 agomir. White arrows indicate the egg granuloma. Scale bar, 100 μm. Insets show a higher magnification of the outlined area. Scale bar, 50 μm. All graph data are expressed as the mean ± SD of at least three biological replicates per group. *P< 0.05, **P< 0.01, ns, not significant. Abbreviation: Sj: S. japonicum; NC: negative control; IHC: immunohistochemical analysis.