Alpha herpesvirus exocytosis from neuron cell bodies uses constitutive secretory mechanisms, and egress and spread from axons is independent of neuronal firing activity
Fig 5
Neuronal activity does not correlate with PRV spread from neurons to non-neuronal cells.
(A) Schematic of modified Campenot tri-chamber. SCG neurons were seeded in the left soma compartment and extended axons under the chamber walls to the right neurite compartment. A detector cell monolayer (PK15) was then added to the neurite compartment. PRV infection was initiated in the soma compartment, and neurons were treated with 4μM TTX, or pulses of 55mM KCl, or untreated, as indicated. (B) Tiled image of neurite chambers (area indicated by red box in panel A). Scale bar represents 1mm. (C) Relative mRFP-VP26 capsid fluorescence of entire neurite chambers over time. (D) Virus titer measured in neurite chambers by serial dilution plaque assay.