Alpha herpesvirus exocytosis from neuron cell bodies uses constitutive secretory mechanisms, and egress and spread from axons is independent of neuronal firing activity
Fig 4
Tetrodotoxin and KCl affect activity of infected neurons.
Primary SCG neurons in modified Campenot trichambers were infected with a recombinant PRV expressing the fluorescent Ca2+ sensor GCaMP3, and treated with 4μM tetrodotoxin (TTX), pulses of 55mM KCl, or untreated, as indicated. (A) Relative Ca2+-dependent GCaMP3 fluorescence of 5 representative cells over time. Relative fluorescence units (RFU) are scaled identically between experimental conditions. (B) Steady-state Ca2+ accumulation indicated by GCaMP3 fluorescence. Scale bar represents 40 μm.