Alpha herpesvirus exocytosis from neuron cell bodies uses constitutive secretory mechanisms, and egress and spread from axons is independent of neuronal firing activity
Fig 3
Some PRV particles colocalize with Rab6a in the proximal axon, but Rab6a is not detected with PRV particles in distal axons.
(A-B) SCG neurons were infected with PRV expressing mCherry-VP26 and EmGFP-Rab6a, and imaged beginning at 5 hr post-infection. Data are representative of 50 cells over 7 independent experiments. Scale bars represent 4 μm. (A) EmGFP-Rab6a vesicles undergo sorting and transport into the proximal axon. Kymograph (inset) represents particle movement along the indicated track (magenta). (B) PRV particles (mCherry-VP26) colocalize with EmGFP-Rab6a in the proximal axon (arrowheads). (C) EmGFP-Rab6a vesicles transport to the distal axon (top set of kymographs), but EmGFP-Rab6a is not detectable on PRV particles (bottom kymographs). Kymographs represent particle movement along the indicated tracks (magenta). Data are representative of ~500 capsid tracks, from 36 independent fields of view, over 4 independent experiments. Scale bar represents 4 μm.