Alpha herpesvirus exocytosis from neuron cell bodies uses constitutive secretory mechanisms, and egress and spread from axons is independent of neuronal firing activity
Fig 2
PRV particles exocytosis is not associated with Rab11a, Rab3a, nor local Ca2+ signaling.
(A-B) SCG neurons were transduced to express mCherry-Rab proteins, infected with PRV expressing gM-pHluorin, and imaged beginning at 5 hr after PRV infection. Images show representative exocytosis events at the moment of exocytosis (time = 0). Each image is 5 μm square. Line plots are ensemble averages of gM-pHluorin (top, green line) and mCherry-Rab protein (bottom, red line) relative fluorescence. Shaded area represents standard deviation. (A) Rab11a is not associated with virus particle exocytosis in neurons. Data represent 41 exocytosis events. (B) Rab3a is not associated with virus particle exocytosis in neurons. Data represent 64 exocytosis events. (C-E) Neurons were infected with PRV expressing gM-pHluorin, loaded with Ca2+ sensitive fluorescent dye Rhod-2-AM, and imaged beginning at 5 hpi. (C) Image is a maximum intensity projection of Rhod-2-AM fluorescence over a 6 min time course. Scale bar represents 4 μm. (D) Rhod-2-AM fluorescence intensity at the region of interest indicated in panel C (black circle) over 6 min time course. (E) Virus particle exocytosis in neurons is not associated with local Ca2+ transients. Ensemble average of gM-pHluorin (top, green line) and Rhod-2-AM (bottom, red line) relative fluorescence over 27 exocytosis events. Shaded area represents standard deviation.