Adaptation of the Mycobacterium tuberculosis transcriptome to biofilm growth
Fig 6
Genome duplication results in complex patterns of gene expression.
A) Experimental diagram highlighting comparator populations: evolved and ancestral biofilm populations. B) Top: large tandem duplication that evolved in populations MT31 and MT55 under selection to grow as a biofilm. Bottom: coordinates of the duplication vary slightly between populations, with an average duplicated length of 175 kb. C) Left: log2 fold changes in gene expression between evolved and ancestral biofilm populations plotted against the position in the genome. Each point is a single gene, colored according to whether that gene lies inside of the duplicated region for that population. Right: Boxplots of log2 fold changes in gene expression, for genes outside (light blue) and inside (dark blue) the duplication. Genes within the duplication were significantly more likely to be upregulated in both populations (Mann Whitney U test with Benjamini-Hochberg correction, p < 0.0001).