Exploiting bacterial effector proteins to uncover evolutionarily conserved antiviral host machinery
Fig 7
Bacterial effector expression or depletion of effector targets enhances oncolytic virus replication in human cancer cells.
A. Representative fluorescence microscopy images (GFP channel) of human 786–0 renal adenocarcinoma cell line infected with VSV-M51R-GFP at 16 hpi. B. Fold-change in normalized viral GFP signal relative to empty vector (EV) control from experiments as in A. Cells were stained with CellTracker Orange dye 16 hpi and imaged to calculate fold-change in normalized GFP signal over EV treatments. Data are means ± SD; n = 3. Statistical significance was determined with unpaired student’s t-test. C. Representative fluorescence microscopy images (GFP channel) of 786–0 cells infected with VSV-M51R-GFP at 16 hpi after transfection with indicated siRNAs. D. Fold-change in normalized viral GFP signal relative to scrambled (control) treatments as in C. Cells were stained with CellTracker dye 16 hpi and imaged to calculate fold-change in normalized GFP signal over control treatments. E. Representative immunoblot of 786–0 whole cell lysate 72 h post-transfection with indicated siRNAs. For B and D: ns = P>0.1234, * = P<0.0332, ** = P<0.0021, *** = P<0.0002, **** = P<0.0001.