Exploiting bacterial effector proteins to uncover evolutionarily conserved antiviral host machinery
Fig 6
Depletion of IpaH4 substrates SHOC2 and PSMC1 enhances arbovirus replication in LD652 cells.
A. Fold-change in normalized viral GFP signals in cells expressing gRNA targeting Relish or SHOC2 relative to empty vector controls 72 hpi. Cells were stained with CellTracker dye 72 hpi and imaged to calculate fold-change in normalized GFP signal over empty vector (control) treatments. Data are means ± SD; n = 3. Statistical significance was determined with unpaired student’s t-test. B. Titer of supernatants from LD652 cell cultures treated as described in A. C. Fold-change in normalized viral GFP signals relative to LacZ siRNA (control) treatments. Cells were stained with CellTracker dye 72 hpi and imaged to calculate fold-change in normalized GFP signal over LacZ (control) siRNA treatments. D. Titer of supernatants from LD652 cell cultures treated as described in C. Data are means ± SD; n = 3. Statistical significance was determined with unpaired student’s t-test; ns = P>0.1234, * = P<0.0332, ** = P<0.0021, *** = P<0.0002, **** = P<0.0001.