The multiplicity of thioredoxin systems meets the specific lifestyles of Clostridia
Fig 3
Trx systems are required for survival to oxidative and nitrosative stresses.
(A, B) Samples were serially diluted, plated in duplicate on TY Taurocholate (Tau) plates and incubated either in anaerobiosis or in hypoxia at 1% O2 for 64 h. Survival was calculated by doing the ratio between CFUs in the last dilution with stress and CFUs in the last dilution without stress. Survival was then normalized as the ratio of the mutant vs the WT. (C, D) Samples were serially diluted and plated on TY and (C) TY + DEA NONOate 750 μM or (D) TY + HClO 0.1% and incubated for 24 h. (E) Samples were incubated in glycylglycine buffer in the presence or absence of H2O2 250 μM for 30 min, serially diluted and plated on TY plates. Mean and SD of survival are shown. Experiments were performed in 5 biological replicates. For all assays, ordinary one-way ANOVA was performed followed by Dunett’s multiple comparison test. The comparisons are made with the WT strain. *: p-value<0.05, ** <0.01, *** <0.001 and **** <0.0001.