Transposon sequencing reveals metabolic pathways essential for Mycobacterium tuberculosis infection
Fig 1
M. tuberculosis Tn mutants in genes encoding central metabolic enzymes exhibit growth defects in Middlebrook 7H9 medium.
Wild-type M. tuberculosis Erdman and M-ES Tn mutant strains were grown in MtbYM rich medium, washed twice in PBS-T, and diluted to OD600 = 0.01 in Middlebrook 7H9 medium. Growth was monitored by measuring the OD600. Tn mutants are grouped according to predicted function: (A) purine and thiamine metabolism (purF, purM, purQ), (B-C) amino acid biosynthesis (pheA, trpB, trpG, proC, gltB, ilvA), (D) pantothenate, riboflavin, and p-amino benzoic acid (PABA) biosynthesis (panC, ribA2, ribG, pabB). Data represent the mean ± standard error of three biological replicates.