Glucuronoxylomannan intranasal challenge prior to Cryptococcus neoformans pulmonary infection enhances cerebral cryptococcosis in rodents
Fig 5
GXM augments the expression of activated RhoA and modulates the cytoskeleton in HBECs in a time-dependent manner.
(A) WB analyses of HBEC lysates stimulated with 10 μg/mL of GXM were performed to assess the expression of activated RhoA protein (GTP-bound) and phosphorylated myosin light chain (pMLC) over the course of 180 min. Quantitative measurements of individual band intensity in WB analyses described in panel A for (B) activated RhoA and (C) phosphor-MLC using LiCOR Image Studio software. Bars represent the means of 3 independent gel results (black circles) and error bars indicate SDs. P-value significance (* P < 0.05, ** P < 0.01 and *** P < 0.001) was calculated using one-way ANOVA and adjusted using the Tukey’s post-hoc analysis. (D) IF images of actin remodeling in HBECs after incubation in the absence (0 min) and the presence of 10 μg/mL GXM for the indicated times (15–180 min). Cells were stained with Phalloidin-594 (red) and DAPI (blue). Arrows indicate actin stress fiber formation; asterisks represent perinuclear relocation of actin. Scale bar, 20 μM.