Human neutrophil IL1β directs intestinal epithelial cell extrusion during Salmonella infection
Fig 5
Caspase-4 regulates cell death in STM-infected PMN-HIOs.
A. Representative fluorescence microscopy images of TUNEL staining of PMN-HIO frozen histology sections. HIOs were microinjected with STM and co-cultured with PMNs in the presence of inhibitors for Caspase-4 (z-LEVD) (20 μM), or DMSO control. B. Quantitation of the percent of lumen filled with TUNEL-positive cells of STM-infected PMN-HIOs with indicated treatments. C. Fluorescent microscopy images of STM-infected PMN-HIO frozen histology sections. Samples were stained for Salmonella (green), E-cadherin (red), and DAPI (blue). D. Quantitation of the number of bacteria per infected cell. E. Quantitation of the percent of infected cells per HIO based on 3 fields per view per HIO. Unless otherwise stated, graphs show the mean +/-SD of n≥ 10 HIOs represented by dots from at least two independent experiments. Outliers were removed using the ROUT method with Q = 0.1%. Significance was determined by unpaired t-test where *p<0.05, **p<0.01.