TMEM41B and VMP1 modulate cellular lipid and energy metabolism for facilitating dengue virus infection
Fig 5
TMEM41B and VMP1 deficiency impose a diverse and distinctive impact on cellular metabolism in 293FT.
(A) Principal Components Analysis (PCA) for the metabolomic datasets of 293FT WT, TMEM41B clone #1, and VMP1 KO clone #1 cells. First two principal components are demonstrated on the x- and y-axis. (B) Heatmap of altered metabolites in the 293FT WT and KO clones. Z-score normalized values of metabolite levels were used to plot the heatmap (see Materials and Methods). Color scheme depicts the relative abundance of metabolites with red and blue indicating higher and lower levels, respectively. Columns indicate different samples, each with 3 replicates. (C) Relative fold changes of metabolites level in TMEM41B KO clone #1 cells in comparison to 293FT WT. Values associated with metabolites from the same family are grouped together in each boxplot. Negative values represent metabolites with decreased abundance in the KO cells, as described in the Materials and Methods. (D) Relative fold changes of metabolites level in VMP1 KO #1 cells in comparison to 293FT WT. Values associated with metabolites from the same family are grouped together in each boxplot. Negative values represent metabolites with decreased abundance in the KO cells, as described in the Materials and Methods.