Reovirus uses temporospatial compartmentalization to orchestrate core versus outercapsid assembly
Fig 5
Core-only factories are spatially segregated from whole virion-containing factories.
(A) Representative SEM image using SEM array tomography sample preparation of an H1299 cell infected with reovirus at an MOI of 3 fixed at 17 hpi. (Green closeup) Distinct region containing cores only. (Blue closeup) Distinct region containing whole viruses. Representative of 8 cells imaged and two independent experiments. (B) H1299 cells were infected with reovirus at an MOI of 3, fixed at 16 hpi and imaged via TEM. Images from various regions around the cell were captured at high magnification and the surface area of viral particles in regions 1–4 were measured using ImageQuant image analysis software. Each point represents a single particle (n = 239). Statistical analysis is reported as a two-tailed student t-test. Data is plotted as mean +/- 95% CI. **** p<0.0001, *** p<0.001, **p<0.05, ns > 0.05. Representative of 18 cells imaged and 4 independent experiments.