Bile acids promote the caveolae-associated entry of swine acute diarrhea syndrome coronavirus in porcine intestinal enteroids
Fig 1
SADS-CoV oral infection leads to a significant increase of bile acids in the small intestine of piglets.
(A) Kinetic of viral shedding in fecal swabs from sucking piglets orally infected with SADS-CoV (n = 6). (B) Principal components analysis (PCA) of the duodenum and ileum from SADS-CoV-infected and mock-infected piglets. (C) KEGG analysis of small intestinal metabolites in SADS-CoV-infected and mock-infected piglets. (D) Volcano plot of small intestinal metabolites in small intestine of SADS-CoV-infected and mock-infected animals. (E) Heatmap of small intestinal metabolites in the duodenum and ileum. (F) Small intestinal bile acid (BA) concentrations of SADS-CoV-infected and mock-infected piglets. P values were determined by unpaired two-tailed Student’s t test. *: p < .05; **: p < .01; ***: p < .001; ns, not significant.