BEX1 is a critical determinant of viral myocarditis
Fig 5
Loss of BEX1 increases susceptibility to multiple viruses ex vivo.
A) qPCR of the CVB3 genome (normalized to Rpl7) from WT and BEX1 KO mouse embryonic fibroblasts (MEFs) infected with CVB3 (MOI = 1) for 24 hours. B-C) Percent of MEFs (WT or BEX1 KO) infected with influenza (B) or Sendai virus (C) after 24 hours, as measured by flow cytometry of GFP-tagged viral capsids. D-F) ELISA assessing IFN-β protein present in growth medium of WT or BEX1 KO MEFs 24 hours after infection with CVB3 (D), influenza (E), or Sendai virus (F). G-I) Rescue of antiviral function in BEX1 KO MEFs by treatment with recombinant IFN-β for cells infected with CVB3 (G), influenza (H), or Sendai virus (I). Statistical analyses by t-test (A-F) and two-way ANOVA (G-I). (# p<0.05 BEX1 KO vs. WT same treatment; * p<0.05 between indicated groups).