Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection
Fig 10
Assembly between major subunit proteins of ESCRT after CSFV infection.
(A, C, E and G) PK-15 cells were infected with CSFV (MOI = 1) for 24 hpi and harvested for immunoprecipitation by using rabbit anti-CHMP2B (A); -CHMP4B (C); -CHMP7 (E) antibody, or mouse anti-VPS4A antibody (G), and the whole-cell lysates were subjected to Western blotting by using the antibodies against HRS, Tsg101, VPS25, CHMP1A, CHMP1B, CHMP2B, CHMP4B, CHMP7, VPS4A and ALIX. These data are representative of three independent experiments. (B, D, F and H) PK-15 cells were infected with CSFV (MOI = 1) for 24 hpi, then fixed and subjected to immunofluorescent by using rabbit anti-CHMP2B (B); -CHMP4B (D) antibody (green) and mouse anti-HRS/VPS4A/Tsg101 antibody (red); or mouse anti-CHMP7 antibody (F) (red) and rabbit anti-Tsg101/VPS4A antibody (green); or mouse anti-VPS4A antibody (H) (red) and rabbit anti-HRS/CHMP2B/CHMP4B/CHMP7 antibody (green). The nuclei were stained with DAPI. Bars = 10 μm. These data are representative of three independent experiments.