Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection
Fig 9
VPS4A protein is closely related to lipid droplets (LDs).
(A) PK-15 cells were infected with CSFV (MOI = 1) for 24 hpi, then fixed and subjected to immunofluorescent by using rabbit anti-ESCRTs antibody (red) and dyeing with BODIPY (green). The nuclei were stained with DAPI. Bars = 10 μm. These data are representative of three independent experiments. (B) The colocalization analysis of ESCRT subunits and LDs was expressed as Pearson’s correlation coefficient. Results are represented as the mean + SD of data from three independent experiments. **, P < 0.01. (C) PK-15 cells were infected with CSFV (MOI = 1) for 24 hpi, then fixed and subjected to immunofluorescent by using rabbit anti-VPS4A/CHMP7 antibody (red), mouse anti-dsRNA (purple), and dyeing with BODIPY (green). The nuclei were stained with DAPI. Bars = 10 μm. These data are representative of three independent experiments. (D) The colocalization analysis of VPS4A or CHMP7, dsRNA, and LDs were expressed as Pearson’s correlation coefficient. The white column indicates the co-localization of the ESCRT subunits and LDs, the black column indicates the co-localization of the ESCRT subunits and dsRNA, and the red column indicates the co-localization of the LDs and dsRNA. Results are represented as the mean + SD of data from three independent experiments.