Structural and antigenic variations in the spike protein of emerging SARS-CoV-2 variants
Fig 5
SARS-CoV-2 RBD mutations that escape antibody binding.
Total escape at each site was measured using a yeast library screening followed by deep sequencing for distinct RBD antibodies (https://jbloomlab.github.io/SARS2_RBD_Ab_escape_maps/). The escape map identifies mutations that escape antibody binding and were mapped on the RBD-Fab structures. The red indicates the site with maximum and white with minimum resistance for antibody binding. (A, C, E, G, I) The structural footprint of C105, C144, C002, C135, and C110 antibodies on the RBM is in red. (B, D, F, H, J) Mapping of the SARS-CoV-2 virus escapes for different antibodies. Mutations that escape antibody binding were broadly located at their binding site in the RBD (see A-B, C-D, E-F, G-H, and I-J pairs). The footprint residues on the RBD have been defined as those residues, which were within 4 Å of a Fab atom. RBD, receptor-binding domain; SARS-CoV-2, Severe Acute Respiratory Syndrome Coronavirus 2.