Genome-scale CRISPR screening reveals that C3aR signaling is critical for rapid capture of fungi by macrophages
Fig 5
C3aR localizes to the early Hc-containing phagosome.
C3aR localizes to Hc-containing phagosomes (A) to a greater extent than latex bead-containing phagosomes (B). BMDMs were infected with the indicated particles (MOI = 5, n = 2 biological replicates per time point). Cells were then stained with a C3aR-specific antibody and imaged using optical sectioning with a confocal microscope. Representative images from a single slice are shown. C. Enlarged views of insets outlined in panels A and B by a white box. Scale bar = 20 μm. D. The mean fluorescence intensity of C3aR in the particle-containing phagosomes was quantified using ImageJ (N>91 phagosomes, **** p<0.0001, **p<0.01 by two-tailed Wilcoxon rank-sum test). The line represents the median phagosomal C3aR intensity.