A revised mechanism for how Plasmodium falciparum recruits and exports proteins into its erythrocytic host cell
Fig 2
Knockdown of HSP101 does not disrupt the stability of the EXP2/PTEX150 subcomplex.
(A), (B), and (C) BN-PAGE analysis of PTEX-HAglmS parasite lines following protein knockdown enables the effects on PTEX complex formation to be examined. Saponin-lysed pellets were lysed with 1% digitonin, separated on 4–12% NativePAGE gel, and analysed via western blotting using PTEX-specific antibodies. Knockdown of EXP2 and PTEX150 prevented the formation of a >1236 kDa PTEX subcomplex. Knockdown of HSP101 in contrast, did not disrupt the ability of PTEX150 and EXP2 to form a >1236kDa complex free of HSP101. * and ** are two non-specific bands observed with rabbit anti-HSP101 antibody. (D) Schematic summarising the impact of knocking down individual PTEX components with regards to complex formation.