A new paradigm for leprosy diagnosis based on host gene expression
Fig 6
Strongest correlations between keratinocyte and EMT-related genes in leprosy lesions.
(A) Heat plot with Spearman’s rho correlation coefficient of the strongest correlations after multiple testing adjustment with at least one gene-pair passing FDR ≤ 0.0001 and rho ≤ -0.8. Correlations with FDR > 0.1 are filled with white. Row colored squares identify gene annotations. Scatter plots of average log2 expression calculated with keratinocyte/epidermal development-related genes previously documented as down-regulated in leprosy skin against dedifferentiation-related genes using either (B) this study RNA-seq dataset or (C) Belone et al. microarray (GSE74481). Lines were drawn based on intercept and beta parameters estimated from robust linear regression for all samples (black line) or separately for PB (blue line), and MB (red line). Spearman’s rho coefficient along with 95% nominal confidence intervals are shown inside scatter plots calculated from all samples. See also S2 and S3 Figs.