A new paradigm for leprosy diagnosis based on host gene expression
Fig 2
Technical and biological validation for selected DEG discovered from RNA sequencing.
(A) Tukey boxplots with RT-qPCR normalized (2–3 reference genes) log2 expression values (A.U) according to clinical and histopathological diagnosis. ODD samples are colored according to M. leprae 16S rRNA qPCR status as positive (blue) or negative (green). (B) log2FC from MB-ODD and PB-ODD comparisons estimated from Bayesian linear mixed models and their 95% credible intervals. (C) Tukey boxplot highlighting IDO1 RT-qPCR normalized log2 expression values by final diagnosis grouped into ODD category. Missing values are omitted.