Cross-neutralization of SARS-CoV-2 by HIV-1 specific broadly neutralizing antibodies and polyclonal plasma
Fig 5
N6 failed to neutralize authentic SARS-CoV-2 virus.
A) SARS-CoV-2 infected Vero-E6 cells monolayer after 72 hours post infection is shown as virus control B) Uninfected Vero-E6 cells monolayer after 72 hours showing complete absence of CPE is shown as representative cell control. Incubation of SARS-CoV-2 virus with 20 μg/ml of C) N6, D) II62 scFv-Fc and E) Anti-RBD IgG respectively, followed by adsorption for 60 minutes on Vero E6 cells. CPE was observed after 72 hours. No significant reduction in the CPE (neutralizing activity) was observed for N6 & II62 mAb which indicates these antibodies did not block SARS-CoV-2 infection. The purified Anti-RBD IgG was used as assay positive control and no CPE was observed (upto 0.1μg/ml) which means virus is completely blocked by neutralizing antibodies and did not show infection on Vero-E6 cells Scale, 500px.