Entamoeba histolytica activation of caspase-1 degrades cullin that attenuates NF-κB dependent signaling from macrophages
Fig 6
Cullin-1/5 are required for NF-κB signaling during Eh-macrophage contact.
(A) THP-1 cells were transfected with 100nM of cullin-1 siRNA and scrambled control (SC siRNA) and in (B) 100nM of cullin-5 siRNA and scrambled control (SC siRNA). After 72h, cells were stimulated with Eh for 1-, 2-, 5- and 10-min. Recombinant human TNF-α (hTNF-α) was used as a positive control for the experiment. Post incubation with Eh, cells were washed and lysed, and an equal amount of lysate was loaded onto the SDS-PAGE gel (12%) and immunoblotted with anti-pIκ-Bα, anti-Iκ-B, anti-p65 and anti-GAPDH antibody. Highlighted boxed areas on the figures show decrease in pIκ-Bα and p65. (C) Protein-protein interaction using STRING v11 showed direct interactions between cullin-1 and Rel and direct interaction between cullin-1/5. Data are representative of two independent experiment.