Elucidating the mechanism by which synthetic helper peptides sensitize Pseudomonas aeruginosa to multiple antibiotics
Fig 7
D-11 enhances the activities of multiple antibiotics ex vivo and in vivo.
(A) Ex vivo bacteremia model for antibiotics, D-11, and their combinations antimicrobial activity. 10 μL of serial decimal dilutions of each one of the combinations of antibiotics with/without D-11 (4 μM) tested in blood were dropped. In vivo efficacy of azithromycin (7.5 mg/kg) (B), chloramphenicol (8 mg/kg) (C), doxycycline (2 mg/kg) (D) and D-11 (10 mg/kg) alone or their combination against P. aeruginosa PAO1 (n = 7 for doxycycline and D-11, n = 8 for the other groups). All the three experiments are using the same group of negative control which treatment with saline. All the data are presented as mean ± SD. ***, P < 0.001; ****, P < 0.0001; ns by Student’s t test.