Human parainfluenza virus type 1 regulates cholesterol biosynthesis and establishes quiescent infection in human airway cells
Fig 6
Reduced HMGCR expression in hPIV1-infected cells.
(A) A549 cells were either mock- or hPIV1-infected at an MOI of 5 and expression levels of HMGCR, SQLE, viral NP and β-actin were assessed by immunoblotting. (B) HPBE cells were either mock- or hPIV1-infected at an MOI of 5 and expression levels of HMGCR, SQLE, viral NP and β-actin were assessed by immunoblotting. (C) A549 cells were either mock-, hPIV1-, or hPIV3-infected at an MOI of 5 and HMGCR and β-actin expression was assessed at late time points by immunoblotting. (D) A549 cells were infected with hPIV1 at an MOI of 5 and HMGCR transcripts were determined by qRT-PCR. HMGCR mRNA levels were normalized to GAPDH mRNA and relative mRNA levels are shown (n = 3). (E) A549 cells infected with hPIV1 at an MOI of 5 were labeled with 35S-Met/Cys for 3 h at 8 dpi and de novo HMGCR synthesis was analyzed by radio-immunoprecipitation.