The establishment of variant surface glycoprotein monoallelic expression revealed by single-cell RNA-seq of Trypanosoma brucei in the tsetse fly salivary glands
Fig 5
Developmental progression of VSG expression.
A. VSG expression data for a random subset of 50 pre-metacyclic cells, with a total VSG UMI count > 10. Data are raw (unscaled) UMI counts. Each column represents a cell and each colour a different VSG. B. Histogram showing the number of VSG expressed for all pre-metacyclic cells (per VSG UMI count > 2, all cells in cluster). C. As for A except that the data are a subset of 50 metacyclic cells with total VSG UMI counts >10. D. Histogram as for B except showing metacyclic VSG transcript diversity (per VSG UMI count >2, all cells in cluster). E. UMAP projection from Fig 2B coloured according to the number of mVSG genes detected per cell.