The establishment of variant surface glycoprotein monoallelic expression revealed by single-cell RNA-seq of Trypanosoma brucei in the tsetse fly salivary glands
Fig 2
InDrop barcoding of salivary gland T. brucei.
A. UMAP projection of two technical replicates (Lib1 and Lib2, red and blue, respectively). B. Merged UMAP projection of technical replicates with clustering information as indicated. C. Gene expression levels for marker genes in the salivary glands, EP1 procyclin (midgut stages) [51]: Tb927.10.10260, BARP (epimastigote stage): Tb927.9.15640 [52], VSG-393: (Genbank) KC612418.1 [48], RBP6: Tb927.3.2930 [12], Calflagin: Tb927.8.5440 [6], HAP2: Tb927.10.10770 [53].