NK cells eliminate Epstein-Barr virus bound to B cells through a specific antibody-mediated uptake
Fig 5
Transfer of B cell bound-EBV particles to NK cells involves contact.
(A) B cells were coated with B95.8 VP, stained for gp350 and co-cultured with NK cells pre-treated for 2h with 20μg/ml of Cytochalasin B or the equivalent volume of vehicle (DMSO). Co-cultures were performed in the presence of EBV S- or S+ serum, with or without 20μg/ml of Cytochalasin B or vehicle for 4h. The proportions of gp350+ B (left) and NK cells (right) corresponding to four experiments are shown. Statistical analysis was performed with Friedman test with Dunn’s multiple comparison test. (B-C) B cells were coated with PKH26-stained B95.8 VP, cultured with CFSE-stained NK cells and EBV S- or S+ for 15min, 1h or 4h, adhered onto coverslips and analyzed by confocal microscopy. (A) Images of Maximum Intensity of the stack of the EBV S- (top) or S+ (bottom) conditions at 15 min. Scale bar: 5μm. Data are representative of three experiments. (B) Quantification of total PKH26 fluorescence per B (left, 31–158 cells per condition) or NK cell (right, 34–116 cells per condition) from two different experiments. Statistical analysis was performed with Mann-Whitney U test.