NK cells eliminate Epstein-Barr virus bound to B cells through a specific antibody-mediated uptake
Fig 2
NK cells in the presence of EBV+ serum remove VP attached to autologous B cells.
(A) B cells were pre-incubated with B95.8 VP for 1h at 4°C, washed, stained with anti-gp350 and cultured alone or with autologous NK cells in the presence of EBV S- or EBV S+ for 4h. The proportions of B cells (CD19+) displaying gp350 were analyzed; representative plots (left) and results of four different experiments (right) are shown. (B) B cells were pre-incubated with AKBM SN, washed and stained with 0.3μM CFSE and for gp350. Samples were cultured alone or with NK cells in the presence of EBV S- or EBV S+ for 4h. The proportions of gp350+ B cells (CFSE+) were analyzed; representative plots (left) and results of four experiments (right) are shown. (C) B cells were pre-incubated with AKBM SN for 1h at 4°C, washed, cultured with NK cells in the presence of EBV S- or EBV S+ for 4h and the proportions of GFP+ B cells (CD19+) were analyzed; representative plots (left) and results of three experiments are shown. (A-C) Statistical analysis was performed with Friedman test with Dunn’s post-test.