The WOPR family protein Ryp1 is a key regulator of gene expression, development, and virulence in the thermally dimorphic fungal pathogen Coccidioides posadasii
Fig 5
Vaccination of mice with C. posadasii Δryp1 mutant.
Protection results for C57BL/6 mice vaccinated with either Δcps1, Δryp1 or a S. cerevisiae culture supernatant (Control). C57BL/6 mice (N = 8 mice/group) were injected either by intraperitoneal (IP) or subcutaneous (SC) injection twice 2 weeks apart with Δcps1, Δryp1, or Control. Vaccinated mice were challenged with a lethal dose of WT (90 spores) and total lung CFUs were determined 14 days post-infection. Δcps1 significantly protects mice from C. posadasii infection compared to Control (P<0.004 both comparisons) while Δryp1 shows no protection (P = 0.99 vs. control). Bar equals geometric mean.