CAR/CXCR5-T cell immunotherapy is safe and potentially efficacious in promoting sustained remission of SIV infection
Fig 5
CAR/CXCR5-T cells continue to expand in vivo.
CAR/CXCR5-T cells expanded at the edges of B cell follicles in vivo at 2 DPT and accumulated within B cell follicles at 6 DPT. (A) Representative image from LN tissue from Rh2850 stained 2 DPT showing CTV-labeled cell proliferation in the extrafollicular (EF) area near the follicular (F) zone. Tissues were stained with anti-CD3 (blue) to label T cells and anti-CD20 (green) to label B cells and delineate B cell follicles (F). The infused T cell product labeled with CTV is pseudo-colored magenta. Cells within F showed lower fluorescence intensity indicating division. Scale bar = 50 μm. (B) Representative image of LN tissue section, from Rh2858 visualized using RNAScope ISH combined with IF, showing expansion of CAR/CXCR5-T cells at the edge of follicles at 2 DPT. The right panel is an enlargement from the left panel showing a cluster of CAR/CXCR5-T cells (red) that appear to be expanding at the edge of the follicle. Tissues were stained with DAPI (blue) and anti-IgM (green) to label B cells and delineate B cell follicles (F), with the more brightly stained germinal center in the center of the F. (C) Representative image of LN tissue from Rh2850, showing CAR/CXCR5-T cell (red) proliferation in F and EF at 6 DPT detected by RNAScope ISH combined with IF. Tissues were stained with anti-IgM (Blue) and anti-Ki67 (green) to mark activation and proliferation. B cell follicles are delineated with white lines. Scale bars = 100 μm. Confocal images were collected using a 20× objective. (D) Percentage of Ki67+ CAR/CXCR5 T cells in the F (green) and EF (blue) areas for each of the T2 animals.