The degree of polymerization and sulfation patterns in heparan sulfate are critical determinants of cytomegalovirus entry into host cells
Fig 5
Mouse CMV entry in sulfotransferase knockout cell lines.
(A) Cells were grown to 90% confluency and infected with wild-type MCMV (strain K181) at an MOI of 3.0. Cells were harvested at 2 hours post-infection, and whole cell lysates were loaded on a polyacrylamide gel for blotting. The blots were probed with anti IE1 antibody. Beta-actin was used as a loading control. (B) Bands from two independent experiments were quantified by densitometry and means were plotted. Standard error of mean was plotted as error bars. Hs6st1 and Hs6st4: Glucosaminyl 6-O-sulfotransferase 1 and 4, respectively. Hs3st1 and Hs3st4: Glucosaminyl 3-O-sulfotransferase 1 and 4, respectively. WT: wild type; dko: double knockout. Data was analyzed by ordinary one way ANOVA with multiple comparisons comparing the means of each test sample with control, and corrected using Dunnett’s post hoc test. Differences were considered significant (*) if p<0.05.