Modern Acinetobacter baumannii clinical isolates replicate inside spacious vacuoles and egress from macrophages
Fig 2
UPAB1 but not 19606 replicates in macrophages.
(A) J774A.1 macrophages were infected with the indicated strains, and after phagocytosis, extracellular bacteria were eliminated with antibiotics. After different time points, total numbers of intracellular CFU were determined. *p < 0.05. (B) Representative images of infected cells at the indicated time points. Samples were stained for cell nuclei (grey), actin (magenta) and Acinetobacter GFP (cyan). Insets show a higher magnification of the area indicated by the white box. Individual channels are presented in S3 Fig. Bars: 20 μm. (C) Quantification of bacteria per vacuole at different times p.i. Each symbol represents an individual vacuole, horizontal line represents the median. At least 200 infected cells were analyzed for each time point for three independent experiments.