Virulence and community dynamics of fungal species with vertical and horizontal transmission on a plant with multiple infections
Fig 2
Shared OTUs between leaf spot fungi and seedborne fungi.
(A) Venn diagram for the number of unique and shared OTUs between leaf spot and seed libraries. The number in parentheses represents the abundance of isolates (for leaf spot fungi, LSF) or sequence reads (for seedborne fungi, SF). The 17 shared OTUs were represented by 311 isolates of leaf spot fungi and 462980 reads of seedborne fungi. (B) Network diagram for the 17 shared OTUs between the leaf spots and the seeds. The lines depict the associations of shared OTUs between leaf spot fungi and seedborne fungi. The abbreviations following each OTU number in the corresponding library represent the family name; Didy, Didymellaceae; Xyle, Xylariales; Nect, Nectriaceae; Pleo, Pleosporaceae; Cera, Ceratobasidiaceae; the numbers in parentheses represent the abundance of isolates (for leaf spot fungi, the number of nonpathogenic fungi is shown in red) or next-generation sequencing reads (for seedborne fungi). Because the ITS sequence obtained by next-generation sequencing technology is short (~250 bp), the alignment is trimmed to this range and clustered to generate novel OTUs. This trimming causes a few OTUs to merge in both libraries, for example, OTU 1_Didy in leaf spot fungi and OTU 125_Xyla in seedborne fungi (see 4.4 Investigation of seedborne fungi of A. adenophora).