pMGF505-7R determines pathogenicity of African swine fever virus infection by inhibiting IL-1β and type I IFN production
Fig 10
ASFV-Δ7R infection induces higher type I IFN and IL-1β production in pigs.
(A-D) Pigs were mock challenged with PBS (Ctrl, n = 4) or challenged intramuscularly with ASFV-Δ7R (Δ7R, n = 5, 103 and 105 HAD50) or parental ASFV-WT (WT, n = 4, 103 HAD50), and the sera samples were collected at day 1, day 5 and day 8 post challenge. The protein levels of IFN-β (A), IFN-α (B) and IL-1β (C) in the serum samples were measured with commercial ELISA kits. The virus loads in the serum samples collected at day 1, day 5 and day 8 post challenge. were evaluated using qPCR. (D) Data presented are mean ± SD. **, P < 0.01, *, P < 0.05, student’s t-test, in comparison with control groups during statistical analysis.