pMGF505-7R determines pathogenicity of African swine fever virus infection by inhibiting IL-1β and type I IFN production
Fig 2
ASFV infection inhibits inducer-mediated IL-1β and IFN-β production in PAMs.
(A-D) PAMs were either mock-infected or infected with ASFV at an MOI of 0.01, 0.1, and 1.0, respectively. At 24 hpi, PAMs were treated with LPS (100 ng/ml) for 8 h and then stimulated with Nigericin (5 μM) for another 4 h (A), or with LPS (100 ng/ml) and poly(dA:dT) (5 μg/ml) for 12 h (B), or with SeV (1 MOI) (C) or poly(dA:dT) (5 μg/ml) (D) for 12 h, then the secretion and the mRNA expression levels of IL-1β were detected by ELISA and qPCR, respectively. A p value of less than 0.05 was considered statistically significant. *p<0.05, **p<0.01, ***p<0.001.