Evolutionary plasticity of SH3 domain binding by Nef proteins of the HIV-1/SIVcpz lentiviral lineage
Fig 6
Comprehensive testing of R-clamp residue pairing rules by mutagenesis of SF2-Nef.
Nef-induced AP-1 activity in cells transfected without Nef (Control) or with the indicated wild-type or mutant versions of SF2 Nef was analyzed as in Fig 1B. The amino acid combinations at positions 83 and 120 of these SF2 Nef variants are indicated as single-letter symbols that are color-coded as explained in Fig 4.